Expi293 Media Composition

The chemically defined formulation results in high reproducibility and lot to lot reliability.

Expi293 media composition. Expi293 met expression medium is a cgmp manufactured chemically defined serum free protein free animal origin free methionine free medium for growth and transfection of expi293f cells. It is based on integrated components including high density expi293f cells in expi293 expression medium and the cationic lipid based expifectamine 293 transfection reagent in combination with specialized transfection enhancers. The expi293 expression system is a complete solution for rapid high yield protein production from mammalian 293 cells. This chemically defined medium does not contain any protein undefined lysates or components of animal origin.

Expi293 expression medium is an optimized chemically defined formulation designed to support the high density culture and transfection of 293 cells e g expi293f cells in suspension. Now there s a better way. Expi293 expression medium contains no human or animal origin components. 10 minutes to read.

Features of the framework include the ability to programmatically append multiple video clips together add video and image. The cells in a suspension culture can be subcultured directly into ex cell 293 from serum supplemented media with little or no adaptation. The medium is not recommended for adherent cell cultures. Switching from 293 to cho cells during drug development doesn t have to create uncertainty.

The components included in the expi293 expression system kit are. The gibco expicho expression system is a completely optimized system consisting of expicho s tm cells that have been adapted to high density serum free suspension culture in expicho tm expression medium along with specially designed transfection reagents and enhancers that. Description expi293 expression medium is a chemically defined serum free protein free medium for growth and transfection of suspension adapted human embryonic kidney hek 293 cells. Two vials of frozen expi293f cells one liter of expi293 expression medium one.

The medium is part of the expi293 platform for rapid high yield transient protein expression from mammalian hek293 cells. Media compositions and editing. 2 vials of frozen gibco expi293f cells. 1 l of gibco expi293 expression medium.

The expi293 expression system brings together a high expressing 293 cell line a chemically defined serum free culture medium and a high efficiency transfection reagent with specialized enhancers. Expi293 expression medium is formulated with glutamax i reagent. Ex cell 293 is an animal protein free serum free medium developed for the long term growth of human embryonic kidney 293 hek 293 and related cells. Production description gibco expi293 expression medium is an optimized chemically defined formulation designed to support the high density culture and transfection of 293 cells e g expi293f cells in suspension.